IONSPRAY MASS-SPECTROMETRY OF MARINE TOXINS .3. ANALYSIS OF PARALYTIC SHELLFISH POISONING TOXINS BY FLOW-INJECTION ANALYSIS, LIQUID-CHROMATOGRAPHY MASS-SPECTROMETRY AND CAPILLARY ELECTROPHORESIS MASS-SPECTROMETRY

被引：55

作者：

PLEASANCE, S ^{[1
]}

AYER, SW ^{[1
]}

LAYCOCK, MV ^{[1
]}

THIBAULT, P ^{[1
]}

机构：

[1] NATL RES COUNCIL CANADA,ATLANTIC REG LAB,INST MARINE BIOSCI,MARINE ANALYT CHEM STAND PROGRAM,HALIFAX B3H 3Z1,NS,CANADA

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 1992年 / 6卷 / 01期

关键词：

D O I：

10.1002/rcm.1290060105

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Ionspray mass spectrometry has been used to monitor the purification of saxitoxin, the parent compound in the family of toxins responsible for paralytic shellfish poisoning (PSP), from a strain of the dinoflagellate Alexandrium excavatum. Quantitative results obtained by flow-injection analysis are compared to those obtained by high-performance liquid chromatography with post-column oxidation and fluorescence detection. The coupling of liquid chromatography and capillary electrophoresis with ionspray mass spectrometry is described for the separation of mixtures of PSP toxins and the highly potent pufferfish toxin tetrodotoxin. Tandem mass spectrometry is used to provide the structural information, and the ability to distinguish isomeric PSP toxins both chromatographically and mass spectrometrically is demonstrated.

引用

页码：14 / 24

页数：11

共 26 条

[1]

[Anonymous], 1990, OFFICAL METHODS ANAL, V15

[2]

BARBER M, 1982, ANAL CHEM, V54, pA645

[3] CHEMICAL ASSAY FOR SAXITOXIN, PARALYTIC SHELLFISH POISON [J].

BATES, HA ;

RAPOPORT, H .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1975, 23 (02) :237-239

[4] ION SPRAY INTERFACE FOR COMBINED LIQUID CHROMATOGRAPHY/ATMOSPHERIC PRESSURE IONIZATION MASS-SPECTROMETRY [J].

BRUINS, AP ;

COVEY, TR ;

HENION, JD .

ANALYTICAL CHEMISTRY, 1987, 59 (22) :2642-2646

[5]

GORDON DC, 1991, 1799 CAN TECH REP FI

[6]

HALL S, 1982, THESIS U ALASKA FAIR

[7] REFERENCE MATERIALS FOR DOMOIC ACID, A MARINE NEUROTOXIN [J].

HARDSTAFF, WR ;

JAMIESON, WD ;

MILLEY, JE ;

QUILLIAM, MA ;

SIM, PG .

FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY, 1990, 338 (04) :520-525

[8]

LAWRENCE JF, 1991, J ASSOC OFF ANA CHEM, V74, P404

[9]

LAWRENCE JF, IN PRESS J ASS OFF A

[10]

LUCKAS B, 1990, Z LEBENSM UNTERS FOR, V1901, P491

← 1 2 3 →