CHEMICAL SYNTHESIS OF A GENE FOR THE PROTEINASE-INHIBITOR EGLIN-C WITHOUT USING T4 DNA-LIGASE AND ITS EXPRESSION IN ESCHERICHIA-COLI

被引：0

作者：

VEIKO, VP ^{[1
]}

OSIPOV, AS ^{[1
]}

SHEKHTER, II ^{[1
]}

BULENKOV, MT ^{[1
]}

RATMANOVA, KI ^{[1
]}

GULKO, LB ^{[1
]}

CHIBISKOVA, NA ^{[1
]}

ERREIS, LL ^{[1
]}

DEREVSHCHIKOVA, EB ^{[1
]}

DEBABOV, VG ^{[1
]}

机构：

[1] MOSCOW GENET & SELECT IND MICROORGANISMS INST,MOSCOW 113545,RUSSIA

来源：

BIOORGANICHESKAYA KHIMIYA | 1995年 / 21卷 / 05期

关键词：

EGLIN C; AMPLIFICATION; EXPRESSION; ESCHERICHIA-COLI;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

A synthetic gene for a proteinase inhibitor (eglin C) that was obtained by direct amplification with oligonucleotides without using DNA ligase and polynucleotide kinase of T4 phage was cloned into expression vectors. A high yield of the polypeptide (110 - 130 mg/l) was attained in E. coli strains.

引用

页码：354 / 358

页数：5

共 39 条

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BIOTECHNOLOGY AND BIOENGINEERING, 1989, 33 (08) : 991 - 998
[2] A LARGE FRAGMENT APPROACH TO DNA-SYNTHESIS - TOTAL SYNTHESIS OF A GENE FOR THE PROTEASE INHIBITOR EGLIN-C FROM THE LEECH HIRUDO-MEDICINALIS AND ITS EXPRESSION IN ESCHERICHIA-COLI
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NUCLEIC ACIDS RESEARCH, 1975, 2 (09) : 1609 - 1620

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