INDUCTION OF TRIPLOID EMBRYOS BY HEAT-SHOCK IN THE CHILEAN NORTHERN SCALLOP ARGOPECTEN-PURPURATUS LAMARCK, 1819

被引:7
作者
TORO, JE
SANHUEZA, MA
PAREDES, L
CANELLO, F
机构
[1] Instituto de Biología Marina, Universidad Austral de Chile, Valdivia
关键词
ARGOPECTEN PURPURATUS; TRIPLOIDY; GENETICS; CHILE;
D O I
10.1080/00288330.1995.9516644
中图分类号
S9 [水产、渔业];
学科分类号
0908 ;
摘要
Triploidy in embryos of the Chilean northern scallop, Argopecten purpuratus Lamarck, 1819, was induced with heat shock. Ploidy levels were assessed by chromosome counting after 3 h from embryos at the 8-16 cell stage of development. The efficiency of induction varied with the time of treatment post-insemination and the temperature of the shock. The duration of the shock showed a positive correlation with the percentage of triploids, the highest of which was obtained using 31 degrees C for 15 min applied 10 min after insemination (66.7 %) but with low percentage of survival embryos after 3 h (26.4 %) and 18 days (11.2 %). The optimal procedure for triploidy induction (62.7 %) and survival after 3 h (62 %) and 18 days of culture (30.5 %) was the heat shock of 31 degrees C for 10 min applied 10 min after fertilisation. We observed no significant difference in growth of shell length between controls and treated larvae after 18 days of culture.
引用
收藏
页码:101 / 105
页数:5
相关论文
共 22 条
[1]  
Allen S.K., Genetic manipulations - critical review of methods and performances for shellfish, Proceedings of the World Symposium on Selection Hibridization and Genetic Engineering in Aquaculture, Bordeaux, 2, pp. 207-217, (1987)
[2]  
Baron J., Diter A., Bodoy A., Triploidy induction in the black scallop (Chlamys varia l.) and its effect on larval growth and survival, Aquaculture, 77, pp. 103-111, (1989)
[3]  
Beaumont A.R., Fairbrother J.E., Ploidy manipulation in molluscan shellfish: A review, Journal of Shellfish Research, 10, pp. 1-18, (1991)
[4]  
Beaumont A.R., Kelly K.S., Production and growth of triploid mytilus edulis larvae, Journal of Experimental Marine Biology and Ecology, 132, pp. 69-84, (1989)
[5]  
Di Salvo L.H., Alarcon E., Martinez E., Uribe E., Progress in mass culture of chlamys (Argopecten) purpuratus lamark (1819) with notes on its natural history, Revista Chilena De Historia Natural, 57, pp. 35-45, (1984)
[6]  
Downing S.L., Allen S.K., Induced triploidy in the pacific oyster, crassostrea gigas: Optimal treatments with cytochalasin b depend on temperature, Aquaculture, 61, pp. 1-15, (1987)
[7]  
Gosling E.M., Nolan A., Triploidy induction by thermal shock in the manila clam, tapes semidecussatus, Aquaculture, 78, pp. 223-228, (1989)
[8]  
Illanes J.E., Cultivo del ostion del norte argopecten purpuratus, Cultivo De Moluscos En America Latina, pp. 211-230, (1990)
[9]  
Komaru A., Uchimura Y., Ieyama H., Wada K.T., Detection of induced triploid scallop, chlamys nobilis, by dna microfluorometry with dapi staining, Aquaculture, 69, pp. 201-209, (1988)
[10]  
Padilla M., Desarrollo larval del ostión chlamys (Argopecten) purpuratus lamark (1819) en condiciones de laboratorio (molluca, pelecypoda), Ciencia Y tecnología Del Mar (CONA), 4, pp. 41-52, (1979)