GENOTOXIC ACTIVITY OF AZIDOTHYMIDINE (AZT) IN IN-VITRO SYSTEMS

被引：31

作者：

CID, MG

LARRIPA, I

机构：

[1] Departamento de Genética, Instituto de Investigaciones Hematológicas Mariano R. Castex, Academia Nacional de Medicina, 1425 Buenos Aires

来源：

MUTATION RESEARCH | 1994年 / 321卷 / 1-2期

关键词：

AZIDOTHYMIDINE (AZT); HUMAN LYMPHOCYTES; CHO CELL LINE; CHROMOSOME DAMAGE;

D O I：

10.1016/0165-1218(94)90127-9

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The genotoxic activity of azidothymidine (AZT) was evaluated in vitro, measuring cytogenetic parameters in two cell systems. In human lymphocytes AZT induced a statistically significant increase in chromosome breakage at 100 mug/ml and in micronucleated cells at the highest dose assayed (500 mug/ml). Sister-chromatid exchanges (SCE) showed a two-fold increase over control values at 50 mug/ml. Lymphocyte cycle kinetics showed an important delay at 500 mug/ml. In Chinese hamster ovary (CHO) cells, AZT produced a significant increase in chromosome aberrations and SCE at 1000 and 500 mug/ml, respectively. At 2500 mug/ml the drug produced a delay in cell cycle progression. These results suggest that AZT is a DNA-damaging agent in both cell systems assayed. Moreover, human lymphocytes seem to be more sensitive to AZT than CHO cells.

引用

页码：113 / 118

页数：6

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