POLYCLONAL IN-111 IGG, I-125 LDL AND I-125 ENDOTHELIN-1 ACCUMULATION IN EXPERIMENTAL ARTERIAL-WALL INJURY

To test iodine-125 labelled low-density lipoprotein (I-125-LDL), polyclonal indium-111 labelled immunoglobulin G (In-111-IgG) and iodine-125 labelled endothelin-1 uptake in metabolically active atheromatous plaques after arterial wall injury, we performed balloon de-endothelialization of carotid arteries or abdominal aortas in 24 New Zealand male rabbits which were fed with a normal diet (n = 14) or a hypercholesterolaemic diet (n = 10) after surgery. Six weeks later the animals were injected with 200 muCi of I-125-LDL and/or with 100 muCi of In-111-IgG or with 9 muCi of I-125-endothelin-1. Forty-eight hours later the animals were sacrificed. Carotid arteries and aortas were removed, counted and fixed for autoradiography and light microscopy examination. Contralateral carotid arteries and thoracic aortas served as controls. Significant In-111-IgG uptake was observed in the injured arteries at autoradiography, with localization mainly in the healing edges, and at well counting. The percentage of the injected dose per gram (%D.inj/g) was 0.0188 +/- 0.06 versus 0.0059 +/- 0.003 in controls (P < 0.05). There was no difference in In-111-IgG uptake between arteries with injury alone and those with active atheroma formation at the site of the injury. Significant I-125-LDL uptake was observed only when lipid deposition was present at light microscopy (%D.inj/g of 0.0024 +/- 0.0005 vs 0.0010 +/- 0.0003 in controls, P < 0.05). I-125-endothelin-1 accumulation was observed in four of five injured aortas both at autoradiography, with diffuse localization, and at well counting (%D.inj/g of 0.0012 +/- 0.0004 in the abdominal aortas vs 0.0008 +/- 0.0003 in the thoracic aortas). Polyclonal IgG may accumulate in injured arteries without active atheroma formation. Inflammatory reaction at the site of the injury may cause In-111-IgG uptake independently of atheromatous plaque formation. LDL accumulation takes place only with active atheroma formation at the site of the injury. Use of labelled peptides such as endothelin-I may provide further insight into the mechanisms of atheromatous plaque formation.