MATHEMATICAL-MODELING OF FIELD-AMPLIFIED SAMPLE INJECTION IN HIGH-PERFORMANCE CAPILLARY ELECTROPHORESIS

被引:107
|
作者
CHIEN, RL
机构
[1] Varian Research Center, Palo Alto, California 94304
关键词
D O I
10.1021/ac00024a012
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
In field-amplified sample injection, the sample is prepared in a low-conductivity buffer and injected into the capillary column containing a high-conductivity buffer. A large amount of sample ions can be injected and concentrated in the column under the enhanced electric field. However, as the low-conductivity buffer migrates electoosmotically into the column, the field strength at the injection point changes continuously during sample injection. A mathematical model to calculate the total amount of injected ions using field-amplified sample injection is presented. Good agreements between theoretical calculations and experimental results are obtained.
引用
收藏
页码:2866 / 2869
页数:4
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