A BETA-D-XYLOSIDASE FROM THE THERMOPHILE CALDOCELLUM-SACCHAROLYTICUM EXPRESSED IN ESCHERICHIA-COLI

被引：11

作者：

LUTHI, E ^{[1
]}

BERGQUIST, PL ^{[1
]}

机构：

[1] UNIV AUCKLAND, CTR GENE TECHNOL, DEPT CELLULAR & MOLEC BIOL, AUCKLAND, NEW ZEALAND

来源：

FEMS MICROBIOLOGY LETTERS | 1990年 / 67卷 / 03期

关键词：

Caldocellum saccharolyticum; Escherichia coli; Thermophile; β-d-Xylosidase;

D O I：

10.1016/0378-1097(90)90011-E

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The gene coding for a β-d-xylosidase (E.C. 3.2.1.37) of the thermophile Caldocellum saccharolyticum was isolated previously as part of a gene cluster which has been cloned in Escherichia coli. The enzyme characteristics were determined in E. coli using toluenized cell extracts. The pH optimum was 6.5 and temperature optimum 70°C. The enzyme was stable at 60°C and the half life at 80°C was 45 minutes. The temperature optimum and the temperature stability exceed those reported for other bacterial or fungal β-d-xylosidases. The enzyme showed no other detectable xylanolytic or cellulolytic enzyme activity. © 1990.

引用

页码：291 / 294

页数：4

共 23 条

[1] BACHMANN SL, 1989, J GEN MICROBIOL, V135, P293
[2] GENETICS AND POTENTIAL BIOTECHNOLOGICAL APPLICATIONS OF THERMOPHILIC AND EXTREMELY THERMOPHILIC MICROORGANISMS
BERGQUIST, PL
LOVE, DR
CROFT, JE
STREIFF, MB
DANIEL, RM
MORGAN, WH
[J]. BIOTECHNOLOGY & GENETIC ENGINEERING REVIEWS, 1987, 5 : 199 - 244
[3] MICROBIAL XYLANOLYTIC SYSTEMS
BIELY, P
[J]. TRENDS IN BIOTECHNOLOGY, 1985, 3 (11) : 286 - 290
[4] EXPRESSION OF LEUCINE GENES FROM AN EXTREMELY THERMOPHILIC BACTERIUM IN ESCHERICHIA-COLI
CROFT, JE
LOVE, DR
BERGQUIST, PL
[J]. MOLECULAR & GENERAL GENETICS, 1987, 210 (03): : 490 - 497
[5] BIOCONVERSION OF HEMICELLULOSE - ASPECTS OF HEMICELLULASE PRODUCTION BY TRICHODERMA-REESEI QM-9414 AND ENZYMIC SACCHARIFICATION OF HEMICELLULOSE
DEKKER, RFH
[J]. BIOTECHNOLOGY AND BIOENGINEERING, 1983, 25 (04) : 1127 - 1146
[6] DELEYN F, 1982, METHOD ENZYMOL, V83, P639
[7] MODE OF ACTION AND PROPERTIES OF XYLANASE AND BETA-XYLOSIDASE FROM NEUROSPORA-CRASSA
DESHPANDE, V
LACHKE, A
MISHRA, C
KESKAR, S
RAO, M
[J]. BIOTECHNOLOGY AND BIOENGINEERING, 1986, 28 (12) : 1832 - 1837
[8] ESTEBAN R, 1983, FEMS MICROBIOL LETT, V17, P163
[9] KERSTERSHILDERSON H, 1982, METHOD ENZYMOL, V83, P631
[10] PURIFICATION AND CHARACTERIZATION OF EXTRACELLULAR BETA-XYLOSIDASE AND BETA-GLUCOSIDASE FROM ASPERGILLUS-FUMIGATUS
KITPREECHAVANICH, V
HAYASHI, M
NAGAI, S
[J]. AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1986, 50 (07): : 1703 - 1711

← 1 2 3 →