A FLUOROMETRIC METHOD OF ANALYSIS FOR TETRACYCLINE

被引：33
作者：
KELLY, RG
PEETS, LM
HOYT, KD
机构：
[1] Department of Pharmacological Research, Toxicology Research Section, Lederle Laboratories, Pearl River
来源：
ANALYTICAL BIOCHEMISTRY | 1969年 / 28卷 / 1-3期
关键词：
D O I：
10.1016/0003-2697(69)90173-0
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A new method of analysis for low concentrations of tetracycline in biological samples is described. The method is dependent upon the extraction of anhydrotetracycline from an acid-treated protein-free filtrate. The anhydrotetracycline, in a chloroform extract, is then treated with ethanolic aluminum chloride to form a highly fluorescent chelate. The procedure described is compared with accepted analytical procedures for tetracycline (microbiological, radiological) in an experiment in which serum concentrations of the antibiotic were determined at various times (hours) following intravenous administration in dogs. © 1969.
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页码：222 / &
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