CAPILLARY ELECTROPHORESIS OF DNA FRAGMENTS IN ENTANGLED POLYMER-SOLUTIONS - A STUDY OF SEPARATION VARIABLES

被引:39
作者
DOLNIK, V [1 ]
NOVOTNY, M [1 ]
机构
[1] INDIANA UNIV,DEPT CHEM,BLOOMINGTON,IN 47405
关键词
CAPILLARY ELECTROPHORESIS; ENTANGLED POLYMER SOLUTIONS; PBR322 DNA HAE-III DIGEST; GALACTOMANNAN; SYNERGEL(TM);
D O I
10.1002/mcs.1220040609
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Capillary electrophoresis (CE) in entangled polymer solutions was studied using pBR322 DNA Hae III digest as a model sample. The influences of polymer nature, monomer chain length, and polymer concentration were investigated. The use of a novel polymer, galactomannan, as the entangled matrix for the separation of DNA fragments has been demonstrated.
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页码:515 / 519
页数:5
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