A PHOSPHATIDYLINOSITOL-SPECIFIC PHOSPHOLIPASE-C FROM CYTOPHAGA - PRODUCTION, PURIFICATION, AND PROPERTIES

被引：2

作者：

ARTURSSON, E ^{[1
]}

PUU, G ^{[1
]}

机构：

[1] NATL DEF RES ESTAB,DIV BIOMED,S-90182 UMEA,SWEDEN

来源：

CANADIAN JOURNAL OF MICROBIOLOGY | 1992年 / 38卷 / 12期

关键词：

PHOSPHATIDYLINOSITOL SPECIFIC; PHOSPHOLIPASE; CYTOPHAGA;

D O I：

10.1139/m92-220

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An extracellular phosphatidylinositol-specific phospholipase C was isolated from a Cytophaga species. The growing conditions were optimized for the production of the enzyme and a purification scheme was developed. The enzyme is small and is not dependent on metal ions for activity. It has a rather low apparent Michaelis constant, 0.2 mM, for phosphatidylinositol and is not stimulated by deoxycholate or extra phospholipid. It releases alkaline phosphatase from rat kidney and cholinesterase from plaice muscle tissue but not acetylcholinesterase from intact rat or pig erythrocytes.

引用

页码：1334 / 1337

页数：4

共 28 条

[1] INCREASED EXTRACELLULAR PRODUCTION OF A CHOLINESTERASE-SOLUBILIZING FACTOR BY CYTOPHAGA-NCMB 1314 DURING MAGNESIUM STARVATION [J].