GSTaxClassifier: a genomic signature based taxonomic classifier for metagenomic data analysis

GSTaxClassifier (Genomic Signature based Taxonomic Classifier) is a program for metagenomics analysis of shotgun DNA sequences. The program includes (1) a simple but effective algorithm, a modification of the Bayesian method, to predict the most probable genomic origins of sequences at different taxonomical ranks, on the basis of genome databases; (2) a function to generate genomic profiles of reference sequences with tri-, tetra-, penta-, and hexa-nucleotide motifs for setting a user-defined database; (3) two different formats (tabular- and tree-based summaries) to display taxonomic predictions with improved analytical methods; and (4) effective ways to retrieve, search, and summarize results by integrating the predictions into the NCBI tree-based taxonomic information. GSTaxClassifier takes input nucleotide sequences and using a modified Bayesian model evaluates the genomic signatures between metagenomic query sequences and reference genome databases.