Antinuclear antibodies

被引:15
作者
Cabiedes, Javier [1 ]
Nunez-Alarez, Carlos A. [1 ]
机构
[1] Inst NAcl Cienacias Med & Nutr Salvador Zubrian, Dept Reumatol, Lab Inmunol, Mexico City, DF, Mexico
来源
REUMATOLOGIA CLINICA | 2010年 / 6卷 / 04期
关键词
Antinuclear antibodies; Indirect immunofluorescence; Autoimmune diseases;
D O I
10.1016/j.reuma.2009.10.004
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Anti-nuclear antibodies (ANA) are immunoglobulin directed against autologous cell nuclear and cytoplasmic components. Besides the autoimmune ANA there are other ANA that can be detected in circulation, like natural and infectious ANA. Because of its high sensibility, detection of the ANA must be done by indirect immunofluorescence (IIF) as screening test and all of those positive samples are convenient to confirm its specificity by ELISA, western blot or other techniques. Positive ANA detected by IIF must be evaluated taking in to account the pattern and titer. The following recommended step is the specificity characterization (reactivity against extractable nuclear antigens [ENA], dsDNA, etc.) which is useful for the diagnosis and follow up of patients with autoimmune diseases, and by such reasoning, its detection must be performed in an orderly and reasonable way using guides or strategies focused to the good use and interpretation of the autoantibodies. The objective of this review is to present a compilation of the literature and our experience in the detection and study of the ANA. (C) 2009 Elsevier Espana, S.L. All rights reserved.
引用
收藏
页码:224 / 230
页数:7
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