DETERMINATION OF A COMPLETE SET OF COUPLING-CONSTANTS IN C-13-LABELED OLIGONUCLEOTIDES

被引：83

作者：

SCHWALBE, H

MARINO, JP

KING, GC

WECHSELBERGER, R

BERMEL, W

GRIESINGER, C

机构：

[1] UNIV FRANKFURT,INST ORGAN CHEM,D-60439 FRANKFURT,GERMANY

[2] YALE UNIV,DEPT CHEM,NEW HAVEN,CT 06511

[3] RICE UNIV,DEPT BIOCHEM & CELL BIOL,HOUSTON,TX 77204

[4] UNIV NEW S WALES,SCH BIOCHEM & MOLEC GENET,KENSINGTON,NSW 2033,AUSTRALIA

[5] BRUKER ANALYT MESSTECH GMBH,D-76287 RHEINSTETTEN,GERMANY

来源：

JOURNAL OF BIOMOLECULAR NMR | 1994年 / 4卷 / 05期

关键词：

J COUPLING CONSTANTS; ISOTOPIC LABELING; RNA; OLIGONUCLEOTIDE STRUCTURE; V(0)-V(4) ANGLE; BETA; EPSILON-ANGLE; HCCH-E COSY; P-FIDS-HSQC;

D O I：

10.1007/BF00404274

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Three experiments are introduced to determine a complete set of coupling constants in RNA oligomers. In the HCCH-E.COSY experiment, the vicinal proton-proton coupling constants can be measured with high accuracy. In the P-FIDS-CT-HSQC experiment, vicinal proton-phosphorus and carbon-phosphorus couplings are measured that depend on the phosphodiester backbone torsion angles beta and epsilon. In the refocussed HMBC experiment, vicinal carbon-proton couplings are measured that depend on the glycosidic torsion angle chi.

引用

页码：631 / 644

页数：14

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