The Hemostatic Effect of Ankaferd Blood Stopper in Rat Bleeding Models with Antithrombotic Drug Therapy: An Experimental In Vivo Study

Objective: Ankaferd blood stopper is a mixture of five medicinal plant extracts used as a hemostatic agent for management of external hemorrhage. The aim of this study was to evaluate the hemostatic effects of Ankaferd blood stopper (ABS) on bleeding after tooth extraction in vivo models with taking antithrombotic drug. Materials and Methods: Forty-eight male Albino Wistar rats were divided into six groups of 8 animals each. Maxillary right first molar tooth of the rats were extracted under general anesthesia. 2 mL saline solutions were applied topically to Control group (group 1), Warfarin group (group 3) and Heparin group (group 5) on the sockets immediately after extraction. Two ml ABS's were applied topically to Ankaferd group (group 2), Warfarin-Ankaferd group (group 4) and Heparin-Ankaferd group (group 6) likewise. The bleeding time and the amount of bleeding were compared among 6 groups just following the tooth extraction. The collected data results were analysed statistically by the ANOVA followed by Tukey test for pair-wise comparisons. Results: The bleeding time was longer in Warfarin and Heparin group than the Control group (p<0.05), Ankaferd, Warfarin-Ankaferd and Heparin-Ankaferd groups (p>0.05). Similiarly, the amount of bleeding of Warfarin group was significantly higher than those of the Control and Warfarin-Ankaferd group (p<0.05). The amount of bleeding were lower in Control, Heparin and Heparin-Ankaferd groups but the differences were not statistically significant (p>0.05). Conclusion: Topically administered ABS is less effective on bleeding control of Warfarin-induced bleeding model than Heparin-induced bleeding model in wistar rats. Resulting small difference in between warfarin and heparin should be investigated in future studies.