PURIFICATION, PROPERTIES, AND SEQUENCE SPECIFICITY OF SSLI, A NEW TYPE-II RESTRICTION ENDONUCLEASE FROM STREPTOCOCCUS-SALIVARIUS SUBSP THERMOPHILUS

被引：17

作者：

BENBADIS, L ^{[1
]}

GAREL, JR ^{[1
]}

HARTLEY, DL ^{[1
]}

机构：

[1] CNRS,ENZYMOL LAB,F-91198 GIF SUR YVETTE,FRANCE

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1991年 / 57卷 / 12期

关键词：

D O I：

10.1128/AEM.57.12.3677-3678.1991

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

SslI, a type II restriction endonuclease, was purified from Streptococcus salivarius subsp. thermophilus strain BSN 45. SslI is an isoschizomer of BstNI. SslI activity was maximum at pH 8.8, 0 to 50 mM NaCl, 2 to 8 mM Mg2+, and 42-degrees-C. Activity against phage DNA in vitro was demonstrated.

引用

收藏

页码：3677 / 3678

页数：2

相关论文

共 19 条

[1] HOST-CONTROLLED MODIFICATION OF BACTERIOPHAGE [J].

ARBER, W .

ANNUAL REVIEW OF MICROBIOLOGY, 1965, 19 :365-+

[2]

BENBADIS L, 1990, BIOCHIMIE, V72, P855, DOI 10.1016/0300-9084(90)90002-X

[3] MULTIPLE MODIFICATION-RESTRICTION SYSTEMS IN LACTIC STREPTOCOCCI AND THEIR SIGNIFICANCE IN DEFINING A PHAGE-TYPING SYSTEM [J].

BOUSSEMAER, JP ;

SCHRAUWEN, PP ;

SOURROUILLE, JL ;

GUY, P .

JOURNAL OF DAIRY RESEARCH, 1980, 47 (03) :401-409

[4] CLEAVAGE SPECIFICITY OF RESTRICTION ENDONUCLEASE ISOLATED FROM HAEMOPHILUS-GALLINARUM (HGA I) [J].

BROWN, NL ;

SMITH, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (08) :3213-3216

[5]

DALY C, 1987, STREPTOCOCCAL GENETI, P259

[6]

DALY C, 1982, MICROBIOLOGY 1982, P213

[7]

ELLIS KJ, 1982, METHOD ENZYMOL, V87, P405

[8] SCRFI - A NEW SEQUENCE-SPECIFIC ENDONUCLEASE FROM STREPTOCOCCUS-CREMORIS [J].

FITZGERLAD, GF ;

DALY, C ;

BROWN, LR ;

GINGERAS, TR .

NUCLEIC ACIDS RESEARCH, 1982, 10 (24) :8171-8179

[9] INVIVO GENETIC EXCHANGE OF A FUNCTIONAL DOMAIN FROM A TYPE-II A METHYLASE BETWEEN LACTOCOCCAL PLASMID PTR2030 AND A VIRULENT BACTERIOPHAGE [J].

HILL, C ;

MILLER, LA ;

KLAENHAMMER, TR .

JOURNAL OF BACTERIOLOGY, 1991, 173 (14) :4363-4370

[10] INTERACTIONS OF BACTERIOPHAGES WITH LACTIC STREPTOCOCCI [J].

KLAENHAMMER, TR .

ADVANCES IN APPLIED MICROBIOLOGY, 1984, 30 :1-29