RAPID SCREENING ASSAY OF CYANIDE CONTENT OF CASSAVA

被引：24

作者：

BRADBURY, JH ^{[1
]}

EGAN, SV ^{[1
]}

机构：

[1] AUSTRALIAN NATL UNIV,DIV BOT & ZOOL,CANBERRA,ACT 2601,AUSTRALIA

来源：

PHYTOCHEMICAL ANALYSIS | 1992年 / 3卷 / 02期

关键词：

CYANIDE CONTENT; CASSAVA; MANIHOT-ESCULENTA; LINAMARIN; CYANOGENIC GLYCOSIDES;

D O I：

10.1002/pca.2800030211

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A rapid, inexpensive screening assay was developed in order to measure the cyanide content of cassava (Manihot esculenta Crantz) tubers. A small disc of parenchyma tissue cut with a cork borer or alternatively grated tissue was placed in a stoppered glass tube and the hydrogen cyanide liberated produced a blue colour on a filter paper previously spotted with a drop of tetra-base 14,4'-methylenebis-(N,N-dimethylaniline)] and cupric acetate. The intensity of the blue colour which developed within one hour was rated visually on a graded scale from 0 to 5. The correlation coefficient between samples accurately analysed for total cyanide and also tested using the rapid assay was 0.77.

引用

页码：91 / 94

页数：4

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