DISTRIBUTION OF CALCIUM-ACTIVATED NEUTRAL PROTEASE INHIBITOR IN THE CENTRAL-NERVOUS-SYSTEM OF THE RAT

被引:18
作者
KAMAKURA, K
ISHIURA, S
IMAJOH, S
NAGATA, N
SUGITA, H
机构
[1] NATL CTR NEUROL & PSYCHIAT,NATL INST NEUROSCI,DIV NEUROMUSCULAR RES,KODAIRA,TOKYO,JAPAN
[2] UNIV TOKYO,INST MED SCI,DIV CELL CHEM,TOKYO 113,JAPAN
关键词
CALCIUM-ACTIVATED NEUTRAL PROTEASE INHIBITOR; CALCIUM-ACTIVATED NEUTRAL PROTEASE; RAT CENTRAL NERVOUS SYSTEM; ABC METHOD;
D O I
10.1002/jnr.490310318
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The ubiquitous existence of calcium-activated neutral protease (CANP, calpain), an enzyme whose activity is regulated by calcium ions and a specific endogenous CANP inhibitor (calpastatin), is well known. Although there has been much investigation concerning the distribution and role of CANP, investigations of the distribution of the CANP inhibitor using immunohistochemical techniques are rare. We made antiserum against a 40K fragment of cDNA corresponding to two C-terminal repeats of rat liver CANP inhibitor expressed in Escherichia coli. Using this antiserum, we examined the distribution of CANP inhibitor in the rat central nervous system by the ABC technique and compared it with the distribution of CANP. Neurons and glias were stained, with the cytosol stained diffusely and the cell membranes stained clearly and strongly. Axons and myelin were stained faintly, but nuclei and vessels were not stained. The distribution of CANP inhibitor was thus found to be similar to that of CANP.
引用
收藏
页码:543 / 548
页数:6
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