IMMUNOHISTOCHEMICAL LOCALIZATION OF LYSYL OXIDASE IN NORMAL HUMAN SKIN

被引：38

作者：

KOBAYASHI, H ^{[1
]}

ISHII, M ^{[1
]}

CHANOKI, M ^{[1
]}

YASHIRO, N ^{[1
]}

FUSHIDA, H ^{[1
]}

FUKAI, K ^{[1
]}

KONO, T ^{[1
]}

HAMADA, T ^{[1
]}

WAKASAKI, H ^{[1
]}

OOSHIMA, A ^{[1
]}

机构：

[1] WAKAYAMA MED COLL,DEPT PATHOL,WAKAYAMA 640,JAPAN

来源：

BRITISH JOURNAL OF DERMATOLOGY | 1994年 / 131卷 / 03期

关键词：

D O I：

10.1111/j.1365-2133.1994.tb08518.x

中图分类号：

R75 [皮肤病学与性病学];

学科分类号：

100206 ;

摘要：

Lysyl oxidase (EC 1.4.3.13), a copper-dependent enzyme which catalyses the formation of aldehyde cross-links, and acts primarily on collagen and elastin, is known to be increased during wound healing and in fibrotic disorders including liver cirrhosis and atherosclerosis, and to be decreased in some hereditary connective tissue diseases and in malignant cell lines. A recent study showed that lysyl oxidase might possess tumour suppressor activity as an antioncogene for ras. Little is known about the localization of this enzyme in human skin. In this study, we determined immunohistochemically the localization of lysyl oxidase in normal skin of young and elderly subjects obtained from sun-exposed and unexposed regions of the body. All skin samples tested had similar distributions of lysyl oxidase. The enzyme was present both extracellularly and intracellularly. Extracellularly, a few granular aggregates of immunoreactants were observed along collagen and elastic fibres. These granules were more common in the adventitial portion of the dermis than in the reticular portion. Of all sun-exposed and unexposed regions studied, the skin of the face displayed the greatest amount of extracellular immunoreactants. Immunopositive granules were observed intracerlularly in fibroblasts, vascular endothelial cells, sweat glands, sebaceous glands, arrector pill muscles and some keratinocytes. These findings provide evidence that, as suggested in recent reports, lysyl oxidase may have a variety of intracellular functions.

引用

页码：325 / 330

页数：6

共 31 条

[1] LOCALIZATION OF HUMAN PLACENTA LYSYL OXIDASE ON HUMAN PLACENTA, SKIN AND AORTA BY IMMUNOELECTRONMICROSCOPY [J].