NUCLEOTIDE-SEQUENCE OF THE PSEUDOMONAS-AERUGINOSA INSERTION-SEQUENCE IS222 - ANOTHER MEMBER OF THE IS3 FAMILY

被引：19

作者：

KROPINSKI, AM ^{[1
]}

FARINHA, MA ^{[1
]}

JANSONS, I ^{[1
]}

机构：

[1] UNIV ALBERTA,DEPT MICROBIOL,EDMONTON T6G 2E1,ALBERTA,CANADA

来源：

PLASMID | 1994年 / 31卷 / 02期

关键词：

D O I：

10.1006/plas.1994.1024

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Sequence analysis of the Pseudomonas aeruginosa insertion sequence element IS222 revealed it to be 1234 bp in size with 23 bp imperfect terminal inverted repeats. Insertion caused a 5-bp duplication of the insertion site. Two ORFS were identified, one of which, ORFA, could encode a basic (pI 10.5) polypeptide with a mass of 11,709. This sequence bears strong homology to the putative ORFA product from the Shigella dysenteriae insertion sequence element IS911, which is a member of the IS3 family of insertion elements. As with other members of this group the nucleotide sequence contains a ''frameshift window'' (AAAAAAG; M. Chandler and O. Fayet (1993). Mol. Microbiol. 7, 497-503) at which ribosome slippage can result in a fusion protein (ORFAB). (C) 1994 Academic Press, Inc.

引用

页码：222 / 228

页数：7

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