A NEW STRATEGY FOR THE CONSTRUCTION OF A TYROSINASE-BASED AMPEROMETRIC PHENOL AND O-DIPHENOL SENSOR

被引：123

作者：

COSNIER, S

INNOCENT, C

机构：

[1] Laboratoire d'Electrochimie Organique et de Photochimie Rédox, URA CNRS 1210, Université Joseph Fourier, 38041 Grenoble Cédex

来源：

BIOELECTROCHEMISTRY AND BIOENERGETICS | 1993年 / 31卷 / 02期

关键词：

D O I：

10.1016/0302-4598(93)80003-D

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel biosensor construction based on the electropolymerization of a pyrrole amphiphilic monomer-tyrosinase (EC 1.14.18.1) mixture previously adsorbed on an electrode is described. The enzyme film was characterized and applied to the detection of several phenols and o-diphenols. The electrode response is based on the amperometric detection of the biocatalytically generated o-quinones. This biosensor has a fast reponse time (about 3 s) and provides very low detection limits (2 nM for catechol). Linear calibration curves were obtained over a wide concentration range. Their slopes, varying from 1 to 300 mA M-1, illustrate the selectivity of the biosensor. The stability, pH and temperature response of the biosensor as well as its kinetic parameter have also been studied.

引用

页码：147 / 160

页数：14

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