VALIDATION OF THE QUANTITATIVE PLANAR CHROMATOGRAPHIC ANALYSIS OF DRUG SUBSTANCES .2. COMPARISON OF SOME PARAMETERS IN OPLC AND TLC

Some performance characteristics of an OPLC purity test have been studied during the development of chromatograms at different flow rates. The parameters investigated were: the linearity of the calibration graph, the limits of detection and quantitation (LOD, LOQ), and the precision of the data. Parameters determined for OPLC developments were compared with those obtained using normal (saturated and unsaturated) chambers. The best results, i.e. the lowest LOD and LOQ as well as the best precision, were obtained by OPLC when eluting with the optimum linear velocity, even though these conditions resulted in the narrowest range over which the calibration was linear.