DETECTION OF BEAN YELLOW MOSAIC-VIRUS IN GLADIOLI CORMS BY THE POLYMERASE CHAIN-REACTION

被引：32

作者：

VUNSH, R ^{[1
]}

ROSNER, A ^{[1
]}

STEIN, A ^{[1
]}

机构：

[1] AGR RES ORG,VOLCANI CTR,DEPT VIROL,POB 6,IL-50250 BET DAGAN,ISRAEL

来源：

ANNALS OF APPLIED BIOLOGY | 1991年 / 119卷 / 02期

关键词：

PCR; BYMV; GLADIOLI CORMS; VIRUS DETECTION;

D O I：

10.1111/j.1744-7348.1991.tb04867.x

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

The polymerase chain reaction (PCR) method readily detected bean yellow mosaic virus (BYMV) in gladioli leaves, but in initial tests PCR did not detect virus in corm tissue. Extracts of RNA from corm tissue were shown to inhibit the amplification of viral sequences when added to a PCR reaction. An additional purification step for the RNA extracts using a Sephadex G-50 column eliminated the inhibitory effect and enabled PCR to amplify and detect viral RNA in corm tissue preparations.

引用

页码：289 / 294

页数：6

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