RECONSTITUTION, A WAY OF BIOCHEMICAL-RESEARCH - SOME NEW APPROACHES TO MEMBRANE-BOUND ENZYMES

被引：227

作者：

RACKER, E

VIOLAND, B

ONEAL, S

ALFONZO, M

TELFORD, J

机构：

[1] Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 1979年 / 198卷 / 02期

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

D O I：

10.1016/0003-9861(79)90521-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A variety of reconstitution procedures has been developed for the analysis of the biological activity of purified membrane proteins. In this paper we describe some new procedures. One is based on the action of two detergents, one ionic and one nonionic. Another method takes advantage of rather unique properties of octylglucoside which, in contrast to the detergents used in the first procedure, allows for virtually instantaneous reconstitution. This procedure is now the method of choice for the reconstitution of bacteriorhodopsin, and the proton pumps of mitochondria or chloroplasts. A third procedure takes advantage of the observation that very short periods of sonication are required when certain solvents (e.g., decane) are present in low concentrations. © 1979.

引用

页码：470 / 477

页数：8

共 19 条

[1]

ALFONZO M, 1979, FED PROC, V38, P455

[2]

BANERJEE R, MEMBRANE BIOCH

[3]

CARROLL RC, 1977, J BIOL CHEM, V252, P6981

[4]

de la HABA G., 1955, JOUR BIOL CHEM, V214, P409