H+/K+-ATPASE CONTENTS OF HUMAN, RABBIT, HOG AND RAT GASTRIC-MUCOSA

被引：11

作者：

ROBERT, JC ^{[1
]}

BENKOUKA, F ^{[1
]}

BAYLE, D ^{[1
]}

HERVATIN, F ^{[1
]}

SOUMARMON, A ^{[1
]}

机构：

[1] ANCIEN HOP BICHAT,INSERM,U10,170 BD NEY,F-75018 PARIS,FRANCE

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1990年 / 1024卷 / 01期

关键词：

(Fundus); (Hog); (Human); (Rabbit); (Rat); ATPase; H[!sup]+[!/sup]/K[!sup]+[!/sup]-; Enzyme activity; Immunological titration; Monoclonal antibody;

D O I：

10.1016/0005-2736(90)90220-I

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A monoclonal antibody (mAb 95-111) was used to titrate the amounts of H+/K+-ATPase in subcellular fractions of the fundus of rats, pigs, rabbits and humans. All four had similar amounts of H+/K+-ATPase: 2.1 ± 0.5 (human), 1.9 ± 0.4 (rabbit), 4.4 ± 0.5 (rat) and 4.2 ± 0.8 (hog) mg ATPase/g wet tissue. The antigen concentrations and H+/K+-ATPase enzymatic activities of subcellular fractions were linearly correlated in all species but rat suggesting that human, rabbit and hog H+/K+-ATPase have similar rates of catalysis (223 μmol Pi/h per mg ATPase). The non-correlation of rat data probably reflects the known lability of the rat enzyme. Hence, immuno-titration promises to be a more reliable method of estimating rat ATPase than the currently used enzymatic assay. The H+/K+-ATPase content of human biopsies was 20-fold higher than previously-published (Smolka, A. and Weinstein, W.M. (1986) Gastroenterology 90, 532-539) suggesting that the previous immuno-titration underestimated the H+/K+-ATPase content of the human fundus. © 1990.

引用

页码：167 / 172

页数：6

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