BINDING-PROPERTIES OF TESTOSTERONE RECEPTORS IN THE HYPOTHALAMIC-PREOPTIC AREA OF THE ADULT MALE-MOUSE BRAIN

被引:6
作者
CLARK, CR
NOWELL, NW
机构
[1] Department of Zoology, The University, Hull
关键词
D O I
10.1016/0039-128X(79)90015-1
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
This study reports the specificity, kinetics and thermodynamics of the binding of tritiated testosterone to specific receptors in the cytosol of the hypothalamic-preoptic area of the adult male mouse brain. Values for the kinetic parameters ka KD, ka, kd and the apparent free energy (ΔGo°c) are reported. The specificity of these receptors was investigated by LH-20 chromatography and sucrosegradient centrifugation. Differences in receptor specificity between the mouse and that reported for the rat are described. The effects of the antiandrogens, cyproterone acetate and BOMT, and the antiestrogens MER-25 and clomiphene citrate on the binding of tritiated testosterone to specific 8s receptors are also reported. The effect of these steroid receptor antagonists on testosterone binding is discussed in relation to the current theory on the mechanism by which androgens influence brain function. © 1979.
引用
收藏
页码:407 / 426
页数:20
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