STUDIES ON FIBER-ENTRAPPED WHOLE MICROBIAL-CELLS IN UREA HYDROLYSIS

被引：6

作者：

GHOSE, TK ^{[1
]}

KANNAN, V ^{[1
]}

机构：

[1] INDIAN INST TECHNOL, INST BIOCHEM ENGN RES, NEW DELHI 110029, INDIA

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 1979年 / 1卷 / 01期

关键词：

D O I：

10.1016/0141-0229(79)90011-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Microbial cells [Micrococcus luteus] possessing high urease activity were entrapped in cellulose triacetate fibers, and their capability to hydrolyze urea was evaluated. The activity obtained using these fibers was dependent on the amount of cell entrapped; the best cells-to-carrier ratio was 1:10. Optimum pH and optimum temperature were 7.2 and 60.degree. C, respectively, for both the free cells and the immobilized cells, which suggests that the cells are physically bound to the polymer matrix. The effect of flow rate on the extent of conversion and continuous hydrolysis of urea was examined. A flow rate corresponding to a residence time of 1.325 h was satisfactory. The computed half-life of the system was 177.7 h. The potential industrial application is also discussed.

引用

页码：47 / 50

页数：4