SPIN FILTER CULTURE - PROPAGATION OF MAMMALIAN CELLS IN SUSPENSION

被引：105
作者：
HIMMELFA.P
THAYER, PS
MARTIN, HE
机构：
[1] Arthur D. Little, Inc., Cambridge
来源：
SCIENCE | 1969年 / 164卷 / 3879期
关键词：
D O I：
10.1126/science.164.3879.555
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
A spin filter device has been used for the propagation in vitro of cells of mouse leukemia L1210 to densities approaching 108 cells per milliliter. By manipulation of flow rates, cells may be exposed to exponentially declining drug concentrations with half-times of 1.5 hours or less, providing a more realistic parallel to in vivo drug treatment than is obtained by other culture methods.
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页码：555 / &
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