PLASMA FACTOR TRIGGERING ALTERNATIVE COMPLEMENT PATHWAY ACTIVATION BY LIPOSOMES

被引：24

作者：

FUNATO, K ^{[1
]}

YAMASHITA, C ^{[1
]}

KAMADA, J ^{[1
]}

TOMINAGA, S ^{[1
]}

KIWADA, H ^{[1
]}

机构：

[1] UNIV TOKUSHIMA,FAC PHARMACEUT SCI,TOKUSHIMA 770,JAPAN

来源：

PHARMACEUTICAL RESEARCH | 1994年 / 11卷 / 03期

关键词：

CETYL ALPHA-D-MANNOSIDE; LIPOSOME; DRUG DELIVERY; ALTERNATIVE COMPLEMENT PATHWAY; COMPLEMENT ACTIVATING FACTOR (CAF);

D O I：

10.1023/A:1018952718496

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Several plasma components, such as complement (C) components, play a role in the clearance of liposomes from the circulation. The interactions between liposomes and the C system were investigated in this study. Multilamellar vesicle (MLV) liposomes, which were damaged by activation of the complement, became susceptible depending on the density of cetylmannoside (Man) on the liposome membrane, and activation proceeded through the alternative C pathway as observed for liposomes without Man (PC-MLV) (K. Funato et al., Biochim. Biophys. Acta 1103:198-204, 1992). In addition, the capacity of Man-modified liposomes (Man-MLV) to activate the alternative C pathway was abolished by preadsorption of plasma with Man-MLV but not with PC-MLV. The results suggest that a specific plasma factor adsorbed with Man-MLV was responsible for the augmentation of the C activation and, further, that the rapid clearance of Man-MLV from the circulation is caused by both enhanced C-mediated liposome permeability and enhanced C-mediated phagocytosis of liposomes.

引用

页码：372 / 376

页数：5

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