Substrate Optimization Using 0.25g substrate of Molasses to produce Biosurfactant by Pseudomonas aeruginosa

Aim: To produce biosurfactants from Pseudomonas aeruginosaon substrate optimization of 0.25g using agricultural resource and to produce Biosurfactants using low cost materials. Study design: Observational Descriptive study Place and duration of study: Study was conducted at Institute of molecular biology and biotechnology in university of Lahore. Duration of the study was two years. Methods: The volume of sample taken 0.25g, of inoculum from growing culture of Pseudomonas aeruginosa was isolated and collected from industrial area of District Kasoor and flasks were than placed into an orbital shaker at speed of 120rpm. The samples were collected in sterile screw capped bottle, aseptically. The samples were stored at 4 degrees C till further use. Results: Surface tension was 53.5, 47.7, 34.5 and 30.8mN/m at time 24, 48, 72 and 96 hours respectively at constant temperature of 37 degrees C and molasses used 0.25g with 1ml inoculum size. The rhamnolipid production was 0.42, 1.95, 2.89 and 3.09 g/L respectively. Similarly the bacterial cell mass was 0.1, 0.5, 1.0 and 1.6 g/L respectively. Conclusion: After optimizing various growth and environmental factors a production of rhamnolipid was achieved.