BIOREACTOR FOR A LARGER SCALE HEPATOCYTE IN-VITRO PERFUSION

被引：153

作者：

GERLACH, JC

ENCKE, J

HOLE, O

MULLER, C

RYAN, CJ

NEUHAUS, P

机构：

[1] FREE UNIV BERLIN,KLINIKUM RUDOLF VIRCHOW,INST CLIN BIOCHEM,D-14050 BERLIN,GERMANY

[2] HAMMERSMITH HOSP,ROYAL POSTGRAD MED SCH,DEPT SURG,LONDON,ENGLAND

来源：

TRANSPLANTATION | 1994年 / 58卷 / 09期

关键词：

D O I：

10.1097/00007890-199411150-00002

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A bioreactor construction for hepatocytes and liver sinusoidal endothelial cells is described. The reactor is based on capillaries for hepatocyte immobilization. Four discrete capillary membrane systems, each serving different purposes, are woven to create a three-dimensional framework for decentralized cell perfusion with low metabolite gradients and decentralized oxygenation and CO2 removal. The biochemical performance of reactors initially seeded with 2.5x10(9) hepatocytes was evaluated over 3 weeks. On day 21, pig albumin synthesis was 4.7 mg/day, lidocaine metabolism was 813.7+/-23 mu g/hr, galactose elimination was 210.1+/-3 mg/hr, and midazolam metabolism was 37.1+/-2 mu g/hr. The specific construction of the reactor enables scale-up to hybrid liver support systems as extracorporeal bridging devices for liver transplantation,

引用

页码：984 / 988

页数：5

共 24 条

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