ENHANCEMENT OF HYDROPEROXIDE-DEPENDENT LIPID-PEROXIDATION IN RAT-LIVER MICROSOMES BY ASCORBIC-ACID

被引：61

作者：

LAUDICINA, DC ^{[1
]}

MARNETT, LJ ^{[1
]}

机构：

[1] WAYNE STATE UNIV, DEPT CHEM, DETROIT, MI 48202 USA

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 1990年 / 278卷 / 01期

关键词：

D O I：

10.1016/0003-9861(90)90233-O

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Simultaneous addition of ascorbic acid and organic hydroperoxides to rat liver microsomes resulted in enhanced lipid peroxidation (~threefold) relative to incubation of organic hydroperoxides with microsomes alone. No lipid peroxidation was evident in incubations of ascorbate alone with microsomes. The stimulatory effect of ascorbate on linoleic acid hydroperoxide (LAHP)-dependent peroxidation was evident at all times whereas stimulation of cumene hydroperoxide (CHP)-dependent peroxidation occurred after a lag phase of up to 20 min. EDTA did not inhibit CHP-dependent lipid peroxidation but completely abolished ascorbate enhancement of lipid peroxidation. Likewise, EDTA did not significantly inhibit peroxidation by LAHP but dramatically reduced ascorbate enhancement of lipid peroxidation. The results reveal a synergistic prooxidant effect of ascorbic acid on hydroperoxide-dependent lipid peroxidation. The inhibitory effect of EDTA on enhanced peroxidation suggests a possible role for endogenous metals mobilized by hydroperoxide-dependent oxidations of microsomal components. © 1990.

引用

页码：73 / 80

页数：8

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[1] AUTOXIDATION OF MICELLES AND MODEL MEMBRANES - QUANTITATIVE KINETIC MEASUREMENTS CAN BE MADE BY USING EITHER WATER-SOLUBLE OR LIPID-SOLUBLE INITIATORS WITH WATER-SOLUBLE OR LIPID-SOLUBLE CHAIN-BREAKING ANTIOXIDANTS [J].