STEROL PATHWAY IN YEAST - IDENTIFICATION AND PROPERTIES OF MUTANT STRAINS DEFECTIVE IN MEVALONATE DIPHOSPHATE DECARBOXYLASE AND FARNESYL DIPHOSPHATE SYNTHETASE

被引：48
作者：
CHAMBON, C
LADEVEZE, V
SERVOUSE, M
BLANCHARD, L
JAVELOT, C
VLADESCU, B
KARST, F
机构：
[1] UNIV POITIERS,BIOCHIM & GENET MICROORGANISMES LAB,40 AVE RECLEUR PINEAU,F-86022 POITIERS,FRANCE
[2] CTR RECH PERNOD RICARD,MICROBIOL LAB,F-94015 CRETEIL,FRANCE
来源：
LIPIDS | 1991年 / 26卷 / 08期
关键词：
D O I：
10.1007/BF02536428
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Yeast mutant strains auxotrophic for ergosterol and blocked in mevalonate diphosphate decarboxylase (erg19) and farnesyl diphosphate (FPP) synthetase (erg20) were isolated. The main feature of the mutants blocked in FPP synthetase is their ability to excrete prenyl alcohols, such as geraniol and farnesol. The isolation of the functional ERG20 gene allowed us to show that farnesyl diphosphate synthetase could be a rate limiting enzyme in ergosterol biosynthesis in yeast.
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页码：633 / 636
页数：4
相关论文
共 12 条
[1]  
ANDERSON MS, 1989, J BIOL CHEM, V264, P19176
[2]   SACCHAROMYCES-CEREVISIAE CONTAINS 2 FUNCTIONAL GENES ENCODING 3-HYDROXY-3-METHYLGLUTARYL-COENZYME-A REDUCTASE [J].
BASSON, ME ;
THORSNESS, M ;
RINE, J .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (15) :5563-5567
[3]   ISOLATION AND PROPERTIES OF YEAST MUTANTS AFFECTED IN FARNESYL DIPHOSPHATE SYNTHETASE [J].
CHAMBON, C ;
LADEVEZE, V ;
OULMOUDEN, A ;
SERVOUSE, M ;
KARST, F .
CURRENT GENETICS, 1990, 18 (01) :41-46
[4]   ISOLATION OF PLEIOTROPIC YEAST MUTANTS REQUIRING ERGOSTEROL FOR GROWTH [J].
KARST, F ;
LACROUTE, F .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1973, 52 (03) :741-747
[5]   ERGOSTEROL BIOSYNTHESIS IN SACCHAROMYCES-CEREVISIAE - MUTANTS DEFICIENT IN EARLY STEPS OF PATHWAY [J].
KARST, F ;
LACROUTE, F .
MOLECULAR & GENERAL GENETICS, 1977, 154 (03) :269-277
[6]   REGULATION OF SQUALENE SYNTHETASE AND SQUALENE EPOXIDASE ACTIVITIES IN SACCHAROMYCES-CEREVISIAE [J].
MBAYA, B ;
FEGUEUR, M ;
SERVOUSE, M ;
KARST, F .
LIPIDS, 1989, 24 (12) :1020-1023
[7]   ISOLATION OF THE ERG12 GENE OF SACCHAROMYCES-CEREVISIAE ENCODING MEVALONATE KINASE [J].
OULMOUDEN, A ;
KARST, F .
GENE, 1990, 88 (02) :253-257
[8]   UNSATURATED FATTY ACID MUTANTS OF SACCHAROMYCES CEREVISIAE [J].
RESNICK, MA ;
MORTIMER, RK .
JOURNAL OF BACTERIOLOGY, 1966, 92 (03) :597-&
[9]   REGULATION OF EARLY ENZYMES OF ERGOSTEROL BIOSYNTHESIS IN SACCHAROMYCES-EEREVISIAE [J].
SERVOUSE, M ;
KARST, F .
BIOCHEMICAL JOURNAL, 1986, 240 (02) :541-547
[10]   ISOLATION AND CHARACTERIZATION OF YEAST MUTANTS BLOCKED IN MEVALONIC ACID FORMATION [J].
SERVOUSE, M ;
MONS, N ;
BAILLARGEAT, JL ;
KARST, F .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1984, 123 (02) :424-430
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