SERUM FROM RATS WITH ACUTE-PANCREATITIS INDUCES EXPRESSION OF THE PAP MESSENGER-RNA IN THE PANCREATIC ACINAR CELL-LINE AR-42J

被引：15

作者：

DUSETTI, NJ ^{[1
]}

MALLO, G ^{[1
]}

DAGORN, JC ^{[1
]}

IOVANNA, JL ^{[1
]}

机构：

[1] INSERM,U315,F-13009 MARSEILLE,FRANCE

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1994年 / 204卷 / 01期

关键词：

D O I：

10.1006/bbrc.1994.2450

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

PAP is a pancreatic secretory protein expressed in the pancreas during the acute phase of pancreatitis. We have investigated the effect of the serum from rats with acute pancreatitis (SAP) on the expression of the PAP mRNA in AR-42J cells. PAP mRNA is strongly induced by SAP in a dose-dependent manner. This induction is abolished by preheating the SAP or diminished by treating the cells with cycloheximide. In addition, amylase but not actin mRNA expression was induced by a different SAP factor. We transfected the AR-42J cells with a chimeric gene containing 1.2 kbp 5'-flanking region of the PAP promoter linked to the CAT reporter gene. The CAT activity was significatively increased in the cells, on treating them with SAP. Our results show : first, SAP contains factors responsible for the PAP mRNA expression and secondly, the cis-acting elements are localized within the 1.2 kbp upstream region of the transcription initiation site. (C) 1994 Academic Press, Inc.

引用

页码：238 / 243

页数：6

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