DETERMINATION OF AFLATOXINS BY REVERSED-PHASE HIGHPERFORMANCE LIQUID-CHROMATOGRAPHY WITH POSTCOLUMN IN-LINE PHOTOCHEMICAL DERIVATIZATION AND FLUORESCENCE DETECTION

被引：78
|
作者：
JOSHUA, H [1 ]
机构：
[1] MERCK & CO INC,RES LABS,RAHWAY,NJ 07065
来源：
JOURNAL OF CHROMATOGRAPHY A | 1993年 / 654卷 / 02期
关键词：
D O I：
10.1016/0021-9673(93)83367-2
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Post-column in-line photochemical derivatization permits fluorescence detection of all four common aflatoxins B-1, B-2, G(1) and G(2). Chromatographic evidence indicates that the photolysis causes the hydration of the non-fluorescent B-1 and G(1) components to the B-2a and G(2a c) omponents respectively. Analysis of naturally contaminated corn samples show no interfering peaks and permits the determination of 1 and 0.25 ppb (w/w) of B-1 and B-2, respectively.
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页码：247 / 254
页数：8
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