TRANSCRIPTIONAL ORGANIZATION OF THE RFAGBIJ LOCUS OF SALMONELLA-TYPHIMURIUM

被引：13

作者：

BRAZAS, R ^{[1
]}

DAVIE, E ^{[1
]}

FAREWELL, A ^{[1
]}

ROTHFIELD, LI ^{[1
]}

机构：

[1] UNIV CONNECTICUT,CTR HLTH,DEPT MICROBIOL,FARMINGTON,CT 06032

来源：

JOURNAL OF BACTERIOLOGY | 1991年 / 173卷 / 19期

关键词：

D O I：

10.1128/jb.173.19.6168-6173.1991

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The transcriptional organization of the rfaGBIJ gene cluster of Salmonella typhimurium was studied by using lacZ and cat transcriptional probes. The results indicated that the leftward end of the gene cluster (rfaG-rfaB-rfaI) is an operon that is transcribed from one or more promoters that lie upstream of rfaG. The results further indicated that the product of the rfaH (sfrB) gene acts as a positive regulator of transcription of the entire rfaGBIJ cluster. At least one site required for the RfaH-mediated transcriptional regulation lies within or very close to the upstream promoter.

引用

页码：6168 / 6173

页数：6

共 22 条

[1] GENETIC-ANALYSIS OF LIPOPOLYSACCHARIDE CORE BIOSYNTHESIS BY ESCHERICHIA-COLI K-12 - INSERTION MUTAGENESIS OF THE RFA LOCUS [J].

AUSTIN, EA ;

GRAVES, JF ;

HITE, LA ;

PARKER, CT ;

SCHNAITMAN, CA .

JOURNAL OF BACTERIOLOGY, 1990, 172 (09) :5312-5325

[2] LINKAGE MAP OF ESCHERICHIA-COLI K-12, EDITION-8 [J].

BACHMANN, BJ .

MICROBIOLOGICAL REVIEWS, 1990, 54 (02) :130-197

[3] SFRA AND SFRB PRODUCTS OF ESCHERICHIA-COLI K-12 ARE TRANSCRIPTIONAL CONTROL FACTORS [J].

BEUTIN, L ;

MANNING, PA ;

ACHTMAN, M ;

WILLETTS, N .

JOURNAL OF BACTERIOLOGY, 1981, 145 (02) :840-844

[4] NUCLEOTIDE-SEQUENCE OF RFAI AND RFAJ GENES ENCODING LIPOPOLYSACCHARIDE GLYCOSYL TRANSFERASES FROM SALMONELLA-TYPHIMURIUM [J].

CARSTENIUS, P ;

FLOCK, JI ;

LINDBERG, A .

NUCLEIC ACIDS RESEARCH, 1990, 18 (20) :6128-6128

[5]

CREEGER ES, 1984, J BIOL CHEM, V259, P3064

[6] INFECTION OF SALMONELLA-TYPHIMURIUM WITH COLIPHAGE-MU D1 (APR LAC) - CONSTRUCTION OF PYR-LAC GENE FUSIONS [J].

CSONKA, LN ;

HOWE, MM ;

INGRAHAM, JL ;

PIERSON, LS ;

TURNBOUGH, CL .

JOURNAL OF BACTERIOLOGY, 1981, 145 (01) :299-305

[7] SUPPRESSION OF THE ABNORMAL PHENOTYPE OF SALMONELLA-TYPHIMURIUM RFAH MUTANTS BY MUTATIONS IN THE GENE FOR TRANSCRIPTION TERMINATION FACTOR RHO [J].

FAREWELL, A ;

BRAZAS, R ;

DAVIE, E ;

MASON, J ;

ROTHFIELD, LI .

JOURNAL OF BACTERIOLOGY, 1991, 173 (16) :5188-5193

[8] REGULATION OF THE F-CONJUGATION GENES STUDIED BY HYBRIDIZATION AND TRA-LACZ FUSION [J].

GAFFNEY, D ;

SKURRAY, R ;

WILLETTS, N .

JOURNAL OF MOLECULAR BIOLOGY, 1983, 168 (01) :103-122

[9] PHAGE LAMBDA GENE-Q ANTITERMINATOR RECOGNIZES RNA-POLYMERASE NEAR THE PROMOTER AND ACCELERATES IT THROUGH A PAUSE SITE [J].

GRAYHACK, EJ ;

YANG, XJ ;

LAU, LF ;

ROBERTS, JW .

CELL, 1985, 42 (01) :259-269

[10] MORPHOLOGICAL HETEROGENEITY AMONG SALMONELLA LIPOPOLYSACCHARIDE CHEMOTYPES IN SILVER-STAINED POLYACRYLAMIDE GELS [J].

HITCHCOCK, PJ ;

BROWN, TM .

JOURNAL OF BACTERIOLOGY, 1983, 154 (01) :269-277

← 1 2 3 →