ALLOSTERIC MODULATION OF THE NMDA RECEPTOR BY DIHYDROLIPOIC AND LIPOIC ACID IN RAT CORTICAL-NEURONS IN-VITRO

被引：46
作者：
TANG, LH
AIZENMAN, E
机构：
[1] Department of Neurobiology University, Pittsburgh School of Medicine Pittsburgh
来源：
NEURON | 1993年 / 11卷 / 05期
关键词：
D O I：
10.1016/0896-6273(93)90115-8
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
The mitochondrial cofactor dihydrolipoic acid (DHLA) was observed to potentiate N-methyl-D-aspartate (NMDA), but not non-NMDA, receptor-mediated whole-cell responses in cultured neurons. This potentiation was readily reversed by the oxidizing agent 5,5'-dithio-bis-(2-nitro-benzoic acid) (DTNB). DHLA was unable to increase NMDA responses previously potentiated by dithiothreitol, nor did it have an effect on NMDA receptors alkylated with N-ethylmaleimide. Single-channel recordings revealed that DHLA produced an increase in NMDA channel open frequency, with no change in single-channel conductance or open time. In contrast, lipoic acid reversed the potentiation of NMDA-evoked responses produced by dithiothreitol and had no effect on NMDA receptors previously oxidized by DTNB. DHLA and lipoic acid are pervasively found substances that readily permeate cellular membranes and thus may influence NMDA receptor activity in vivo by modifying its redox site.
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页码：857 / 863
页数：7
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