SEQUENCES AT THE SOMATIC RECOMBINATION SITES OF IMMUNOGLOBULIN LIGHT-CHAIN GENES

被引：833
作者：
SAKANO, H
HUPPI, K
HEINRICH, G
TONEGAWA, S
机构：
[1] Basel Institute for Immunology, CH-4005 Basel
来源：
NATURE | 1979年 / 280卷 / 5720期
关键词：
D O I：
10.1038/280288a0
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The entire nucleotide sequence of a 1.7-kilobase embryonic DNA fragment containing five joining (J) DNA segments for mouse immunoglobulin [~amp]kappa; chain gene has been determined. Each J DNA segment can encode amino acid residues 96-108. Comparison of one of the five J DNA sequences with those of an embryonic variable (V) gene and a complete [~amp]kappa; chain gene permitted localisation of a precise recombination site. The 5[~amp]prime;-flanking regions of J DNA segments could form an inverted stem structure with the 3[~amp]prime;-non-coding region of embryonic V genes. This hypothetical structure and gel-blotting analysis of total embryo and myeloma DNA suggest that the somatic recombination may be accompanied by excision of an entire DNA segment between a V gene and a J DNA segment. Antibody diversity may in part be generated by modulation of the precise recombination sites. © 1979 Nature Publishing Group.
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页码：288 / 294
页数：7
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