ENTAMOEBA-HISTOLYTICA - CYTOPATHOGENICITY OF INTACT AMEBAE AND CELL-FREE-EXTRACTS - ISOLATION AND CHARACTERIZATION OF AN INTRACELLULAR TOXIN

被引：42
作者：
BOS, HJ
机构：
[1] Department of Parasitology, Medical Center, University of Leiden, Leiden
来源：
EXPERIMENTAL PARASITOLOGY | 1979年 / 47卷 / 03期
关键词：
Axenic; baby hamster; cell-free; Cytopathogenicity; Entamoeba histolytica; Extract; Gel chromatography; Kidney cells; parasitic; Protozoa; Serum; Toxin; Virulence;
D O I：
10.1016/0014-4894(79)90089-4
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
Toxicity assays on cell-free extracts of virulent and nonvirulent strains of Entamoeba histolytica were carried out in microtiter plates. These extracts had a cytopathogenic effect (CPE) on monolayers of baby hamster kidney cells. CPE was inhibited by normal human serum, fetal calf serum, and other sera, probably due to their IgG component. Using gel chromatography the toxic material, a protein, was found in a fraction with molecular weight between 35,000 and 45,000. This fraction contained a strong glycoprotein antigen. CPE caused by this toxin differs in several ways from the earlier described contact lysis" caused by intact amebae. The possible significance of these two modes of toxicity of Entamoeba histolytica for the pathogenesis of amebiasis is discussed. © 1979."
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页码：369 / 377
页数：9
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