TIME AND FREQUENCY-DOMAIN ANALYSIS OF NMR DATA COMPARED - AN APPLICATION TO 1D H-1 SPECTRA OF LIPOPROTEINS

A comparison between a time domain analysis algorithm (VARPRO) and a frequency domain analysis algorithm (FITPLA(C)) for parameter estimation of magnetic resonance spectroscopy (MRS) data series is presented. VARPRO analyses the measured MRS signal (free induction decay; FID); FITPLA(C) analyses the discrete Fourier transform of the FID, the frequency domain magnetic resonance spectrum. A rapid time domain method, used to subtract the dominating water resonance from a H-1 MRS FID, without affecting the metabolites of interest, is outlined and applied. Also a new ''pseudo-frequency selective'' approach to time domain fitting is introduced. The possibilities of combining the most favorable features of time and frequency domain processing into one single MRS signal processing method are assessed. The H-1 MRS signals of ultracentrifuged very low (VLDL), intermediate (IDL), and high (HDL) density lipoprotein fractions from human blood plasma were used for the comparisons. The results from both algorithms were in good agreement.