SIGMA-FACTOR AND SPORULATION GENES IN CLOSTRIDIUM

被引:32
作者
SAUER, U [1 ]
SANTANGELO, JD [1 ]
TREUNER, A [1 ]
BUCHHOLZ, M [1 ]
DURRE, P [1 ]
机构
[1] UNIV GOTTINGEN, INST MIKROBIOL, D-37077 GOTTINGEN, GERMANY
关键词
CLOSTRIDIUM; DIFFERENTIATION; GENE EXPRESSION; SIGMA FACTOR; SPORULATION; TRANSCRIPTIONAL REGULATION;
D O I
10.1016/0168-6445(95)00005-W
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The genus Clostridium, represented by Gram-positive, anaerobic, spore-forming bacteria, is well known for its clinical importance and considerable biotechnological potential. Recently, evidence for a functional role of the transcription factors sigma(A), sigma(E), sigma(G), and sigma(K) in this genus was provided by cloning and sequencing these genes from C. acetobutylicum. In C. kluyveri, a partially sequenced open reading frame was found to encode the N terminus of the putative sigma factor L with significant similarity to members of the sigma(54) family. The identification of sequences with high similarity to the Bacillus sigma(F) (C. acetobutylicum), sigma(H) (several clostridial species), and sigma(D) (C. thermocellum)-controlled consensus promoters renders the existence of these transcription factors in clostridia very likely. These data are in agreement with information obtained by RNA transcript mapping (sigma(A), sigma(H)) heterologous DNA hybridization (sigma(D), sigma(H)), and immuno characterization of purified proteins (sigma(A)) from various clostridial species. Thus, the picture emerges that a fundamental similarity exists at the genetic level between the regulation of various cellular responses, in particular sporulation, in the genera Bacillus and; Clostridium. The different induction patterns of sporulation in Bacillus spp. (nutrient starvation) and many clostridial species (cessation of growth or exposure to oxygen in the presence of excess nutrients) are most interestingly not reflected in the general regulatory features of this developmental process.
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页码:331 / 340
页数:10
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