ORGANIZATION AND SEQUENCE-ANALYSIS OF THE 5S RIBOSOMAL-RNA GENES IN BRASSICA-CAMPESTRIS

被引：9
作者：
BHATIA, S
SINGH, K
JAGANNATHAN, V
LAKSHMIKUMARAN, M
机构：
[1] TATA ENERGY RES INST,DIV BIOTECHNOL,90 JOR BAGH,NEW DELHI 110003,INDIA
[2] TAMIL NADU AGR UNIV,CTR PLANT MOLEC BIOL,COIMBATORE 641003,TAMIL NADU,INDIA
来源：
PLANT SCIENCE | 1993年 / 92卷 / 01期
关键词：
5S RDNA; TANDEM REPEAT; BRASSICA; METHYLATION;
D O I：
10.1016/0168-9452(93)90065-8
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The 5S rRNA gene from Brassica campestris has been cloned, sequenced and characterized. The 5S rDNA repeat unit is 495 bp in length. It consists of a highly conserved, 119 bp coding region and a variable non-coding spacer region which separates it from the coding region of the next repeat unit. The 5S rDNA units are organized into clusters of tandem repeats. Sequences responsible for initiation and termination of transcription of the 5S rRNA are present within the repeat unit. Hybridization data with isoschizomers reveals a high amount of 'C' methylation. The size of the repeat unit and the organization of the 5S rRNA genes in other closely related crucifers has been investigated and found to be quite similar to that of B. campestris.
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页码：47 / 55
页数：9
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