FLUORESCENCE OF COMPLEXES OF QUINACRINE MUSTARD WITH DNA .2. DEPENDENCE ON THE STAINING CONDITIONS

被引：9

作者：

ANDREONI, A

COVA, S

BOTTIROLI, G

PRENNA, G

机构：

[1] UNIV PAVIA,CTR STUDIO ISTOCHIM,IST ANAT COMPARATA,I-27100 PAVIA,ITALY

[2] POLITECN MILAN,IST FIS,I-20133 MILAN,ITALY

来源：

PHOTOCHEMISTRY AND PHOTOBIOLOGY | 1979年 / 29卷 / 05期

关键词：

D O I：

10.1111/j.1751-1097.1979.tb07797.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Abstract—Measurements of time‐resolved fluorescence of Micrococcus lysodeikticus DNA stained with quinacrine mustard are presented in this paper. In particular, the dependence of the fluorescence decay law both on the degree of DNA saturation by the dye and on the ionic strength of the solutions is investigated. It is shown that the quinacrine mustard molecules intercalating the DNA base pairs exhibit two different fluorescence behaviors which make them either donors or acceptors in a Forster energy transfer mechanism. A mathematical formulation of this mechanism allows us to interpret all the experimental fluorescence decay curves. In addition, some new properties of the external binding mode are obtained. Copyright © 1979, Wiley Blackwell. All rights reserved

引用

页码：951 / 957

页数：7

共 15 条

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GALANIN MD, 1955, SOV PHYS JETP-USSR, V1, P317

[12] PROCEDURE FOR ISOLATION OF DEOXYRIBONUCLEIC ACID FROM MICRO-ORGANISMS [J].