3-DIMENSIONAL OBSERVATION OF WHOLE-MOUNT CULTURED-CELLS STAINED WITH HISTOCHEMICAL REACTIONS BY ULTRAHIGH VOLTAGE ELECTRON-MICROSCOPY

被引：0
作者：
NAGATA, T
机构：
来源：
CELLULAR AND MOLECULAR BIOLOGY | 1995年 / 41卷 / 06期
关键词：
HIGH VOLTAGE ELECTRON MICROSCOPY; HISTOCHEMISTRY; 3D OBSERVATION; CELL ORGANELLES; THICK SPECIMENS;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Thick biological specimens prepared as whole mount cultured cells stained with histochemical reactions, such as thiamine pyrophosphatase, glucose-6-phosphatase, cytochrome oxidase, acid phosphatase, DAB reactions demonstrating specific cell organelles such as Golgi apparatus, endoplasmic reticulum, mitochondria, lysosomes, peroxisomes and pinocytotic vesicles, were observed by ultrahigh voltage electron microscopy at accelerating voltages of 400-1000 kV producing stereo-pairs. As a result, those cell organelles were observed 3-dimensionally and the relative relationships between these organelles demonstrated.
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页码：783 / 792
页数：10
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