A BIO-FET SENSOR FOR LACTOSE BASED ON CO-IMMOBILIZED BETA-GALACTOSIDASE GLUCOSE-DEHYDROGENASE

被引：22

作者：

SEVILLA, F

KULLICK, T

SCHEPER, T

机构：

[1] UNIV MUNSTER,INST BIOCHEM,WILHELM KLEMM STR 2,D-48149 MUNSTER,GERMANY

[2] UNIV ST TOMAS ESPANA,NAT SCI RES CTR,MANILA 1008,PHILIPPINES

[3] UNIV HANNOVER,INST TECH CHEM,D-30167 HANNOVER,GERMANY

来源：

BIOSENSORS & BIOELECTRONICS | 1994年 / 9卷 / 4-5期

关键词：

BIOFET SENSOR; ENZYME FET SENSOR; LACTOSE SENSOR; CO-IMMOBILIZED BETA-GALACTOSIDASE GLUCOSE DEHYDROGENASE;

D O I：

10.1016/0956-5663(94)80024-3

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A field-effect transistor (FET) biosensor for lactose is presented. The bioFET device employs a bi-enzyme system consisting of beta-galactosidase and glucose dehydrogenase co-immobilized on the surface of the FET gate. The sensor response was measured in a differential mode, wherein the source voltage in the bioFET is compared with that in a pH-sensitive FET sensor placed next to it. The sensor produced a rapid and reproducible response to lactose. The response was affected by several factors such as stirring, NAD+ concentration, pH and buffer capacity. The optimum conditions for the performance of the bioFET were investigated.

引用

页码：275 / 281

页数：7

共 10 条

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