REGULATION OF METALLOTHIONEIN THYMIDINE KINASE FUSION PLASMIDS INJECTED INTO MOUSE EGGS

被引：238

作者：

BRINSTER, RL ^{[1
]}

CHEN, HY ^{[1
]}

WARREN, R ^{[1
]}

SARTHY, A ^{[1
]}

PALMITER, RD ^{[1
]}

机构：

[1] UNIV WASHINGTON, DEPT BIOCHEM, HOWARD HUGHES MED INST LAB, SEATTLE, WA 98195 USA

来源：

NATURE | 1982年 / 296卷 / 5852期

关键词：

D O I：

10.1038/296039a0

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A plasmid was constructed with the promoter/regulatory region of the mouse metallothionein-I gene fused to the structural gene of herpes virus thymidine kinase. When mouse eggs were microinjected with this plasmid and incubated with Cd (a natural inducer of metallothionein gene transcription) thymidine kinase activity increased .apprx. 10-fold compared with control eggs not exposed to Cd. Analysis of a set of deletion mutants revealed that the minimum sequence required for Cd regulation lies within 90 nucleotides of the transcription start site.

引用

页码：39 / 42

页数：4

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