AN AFFINITY PURIFIED OVINE ANTIVENOM FOR THE TREATMENT OF VIPERA-BERUS ENVENOMING

被引：69

作者：

SMITH, DC

REDDI, KR

LAING, G

THEAKSTON, RGD

LANDON, J

机构：

[1] ST BARTHOLOMEWS HOSP,SCH MED,DEPT CHEM PATHOL,CHARTERHOUSE SQ,LONDON EC1,ENGLAND

[2] ST BARTHOLOMEWS HOSP,SCH MED,THERAPEUT ANTIBODIES INC,LONDON EC1,ENGLAND

[3] UNIV LIVERPOOL LIVERPOOL SCH TROP MED,LIVERPOOL L3,ENGLAND

来源：

TOXICON | 1992年 / 30卷 / 08期

关键词：

D O I：

10.1016/0041-0101(92)90384-H

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

A novel antivenom for treating patients with Vipera berus bite has been developed. Sheep are immunised monthly with relatively small amounts of Vipera berus (common adder) venom and the resultant antisera pooled. The immunoglobulin fraction is precipitated with sodium sulphate then cleaved with papain to produce Fab fragments. Finally, those Fab fragments that are directed specifically against components in the venom are purified by affinity chromatography on columns comprising V. berus venom coupled to cyanogen bromide activated Sepharose 4B. The resultant product is some three times more effective than the non-purified Fab in protecting mice against the lethal venom effects.

引用

页码：865 / 871

页数：7