EXOTOXIN-A OF PSEUDOMONAS-AERUGINOSA - EVIDENCE THAT DOMAIN-I FUNCTIONS IN RECEPTOR-BINDING

被引：34
作者：
GUIDIRONTANI, C
COLLIER, RJ
机构：
[1] HARVARD UNIV,SCH MED,DEPT MICROBIOL & MOLEC GENET,BOSTON,MA 02115
[2] SHIPLEY INST MED,BOSTON,MA 02115
来源：
MOLECULAR MICROBIOLOGY | 1987年 / 1卷 / 01期
关键词：
D O I：
10.1111/j.1365-2958.1987.tb00528.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have constructed defined deletions in the structural gene of Pseudomonas aeruginosa exotoxin A (ETA) In order to probe the function of Domain t of this protein. Three forms of the gene containing specific deletions were expressed in a strain of Escherichia coli K12 with lesions in the htpR and ion genes; extracts containing the gene products were tested for ADP‐ribosylation activity, cytotoxicity, and ability to protect sensitive cells from the cytotoxic action of authentic ETA. Two of the mutant ETAs gave concentration‐dependent protection against authentic ETA, and protection correlated with the presence of the bulk of Domain I. The results support the notion that Domain I functions in binding the toxin to specific cell‐surface receptors. Copyright © 1987, Wiley Blackwell. All rights reserved
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页码：67 / 72
页数：6
相关论文
共 19 条
[1]   STRUCTURE OF EXOTOXIN-A OF PSEUDOMONAS-AERUGINOSA AT 3.0-ANGSTROM RESOLUTION [J].
ALLURED, VS ;
COLLIER, RJ ;
CARROLL, SF ;
MCKAY, DB .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (05) :1320-1324
[2]  
BIRNBOIM HC, 1979, NUCLEIC ACIDS RES, V7, P1513
[3]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[4]  
CARROLL SF, 1987, IN PRESS J BIOL CHEM
[5]   ENZYMATICALLY ACTIVE PEPTIDE FROM ADENOSINE DIPHOSPHATE RIBOSYLATING TOXIN OF PSEUDOMONAS-AERUGINOSA [J].
CHUNG, DW ;
COLLIER, RJ .
INFECTION AND IMMUNITY, 1977, 16 (03) :832-841
[6]   VIBRIO-CHOLERAE CHOLERAGENOID - MECHANISM OF INHIBITION OF CHOLERA TOXIN ACTION [J].
CUATRECA.P .
BIOCHEMISTRY, 1973, 12 (18) :3577-3581
[7]   ELECTROPHORETIC ANALYSIS OF MAJOR POLYPEPTIDES OF HUMAN ERYTHROCYTE MEMBRANE [J].
FAIRBANKS, G ;
STECK, TL ;
WALLACH, DFH .
BIOCHEMISTRY, 1971, 10 (13) :2606-+
[8]  
FALMAGNE P, BACTERIAL PROTEIN TO, P3
[9]   CLONING, NUCLEOTIDE-SEQUENCE, AND EXPRESSION IN ESCHERICHIA-COLI OF THE EXOTOXIN-A STRUCTURAL GENE OF PSEUDOMONAS-AERUGINOSA [J].
GRAY, GL ;
SMITH, DH ;
BALDRIDGE, JS ;
HARKINS, RN ;
VASIL, ML ;
CHEN, EY ;
HEYNEKER, HL .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (09) :2645-2649
[10]   FUNCTIONAL DOMAINS OF PSEUDOMONAS EXOTOXIN IDENTIFIED BY DELETION ANALYSIS OF THE GENE EXPRESSED IN ESCHERICHIA-COLI [J].
HWANG, J ;
FITZGERALD, DJ ;
ADHYA, S ;
PASTAN, I .
CELL, 1987, 48 (01) :129-136
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