2 DISTINCT YEAST TRANSCRIPTIONAL ACTIVATORS REQUIRE THE FUNCTION OF THE GCN5 PROTEIN TO PROMOTE NORMAL LEVELS OF TRANSCRIPTION

被引:266
作者
GEORGAKOPOULOS, T
THIREOS, G
机构
[1] Inst Molec Biol and Biotechnology, Foundation Research and Technology, Heraklion 711 10, Crete
关键词
GCN4; HAP2-HAP3-HAP4;
D O I
10.1002/j.1460-2075.1992.tb05507.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
When yeast cells are grown under conditions of amino acid limitation, transcription of amino acid biosynthetic genes is increased through the action of the GCN4 transcriptional regulator. gcn5 mutant strains exhibit poor growth under such conditions. We have established that GCN4 requires the function of GCN5 in order to promote normal levels of transcriptional activation. In addition, we have shown that GCN5 is also required for the activity of the HAP2-HAP3-HAP4 transcriptional activation complex, which mediates the transcription of genes involved in respiratory functions. Thus, GCN5 is a new member of the recently revealed general class of transcriptional regulators that collaborate with certain specific DNA binding activators to promote high levels of transcription. We have cloned and sequenced the GCN5 gene. The deduced GCN5 protein contains a region conserved in other yeast, Drosophila and human proteins, all members of this new class of transcriptional activators.
引用
收藏
页码:4145 / 4152
页数:8
相关论文
共 57 条
[1]   SUPPRESSION OF RIBOSOMAL REINITIATION AT UPSTREAM OPEN READING FRAMES IN AMINO ACID-STARVED CELLS FORMS THE BASIS FOR GCN4 TRANSLATIONAL CONTROL [J].
ABASTADO, JP ;
MILLER, PF ;
JACKSON, BM ;
HINNEBUSCH, AG .
MOLECULAR AND CELLULAR BIOLOGY, 1991, 11 (01) :486-496
[2]   MOLECULAR ANALYSIS OF SNF2 AND SNF5, GENES REQUIRED FOR EXPRESSION OF GLUCOSE-REPRESSIBLE GENES IN SACCHAROMYCES-CEREVISIAE [J].
ABRAMS, E ;
NEIGEBORN, L ;
CARLSON, M .
MOLECULAR AND CELLULAR BIOLOGY, 1986, 6 (11) :3643-3651
[3]   STRUCTURE AND FUNCTION OF THE TRP3 GENE OF SACCHAROMYCES-CEREVISIAE - ANALYSIS OF TRANSCRIPTION, PROMOTER SEQUENCE, AND SEQUENCE CODING FOR A GLUTAMINE AMIDOTRANSFERASE [J].
AEBI, M ;
FURTER, R ;
PRANTL, F ;
NIEDERBERGER, P ;
HUTTER, R .
CURRENT GENETICS, 1984, 8 (03) :165-172
[4]   GCN4 PROTEIN, A POSITIVE TRANSCRIPTION FACTOR IN YEAST, BINDS GENERAL CONTROL PROMOTERS AT ALL 5' TGACTC 3' SEQUENCES [J].
ARNDT, K ;
FINK, GR .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (22) :8516-8520
[5]   YEAST UPSTREAM ACTIVATOR PROTEIN GCN4 CAN STIMULATE TRANSCRIPTION WHEN ITS BINDING-SITE REPLACES THE TATA ELEMENT [J].
CHEN, W ;
STRUHL, K .
EMBO JOURNAL, 1989, 8 (01) :261-268
[6]   A YEAST AND A HUMAN CCAAT-BINDING PROTEIN HAVE HETEROLOGOUS SUBUNITS THAT ARE FUNCTIONALLY INTERCHANGEABLE [J].
CHODOSH, LA ;
OLESEN, J ;
HAHN, S ;
BALDWIN, AS ;
GUARENTE, L ;
SHARP, PA .
CELL, 1988, 53 (01) :25-35
[7]   THE SPT6 GENE IS ESSENTIAL FOR GROWTH AND IS REQUIRED FOR DELTA-MEDIATED TRANSCRIPTION IN SACCHAROMYCES-CEREVISIAE [J].
CLARKADAMS, CD ;
WINSTON, F .
MOLECULAR AND CELLULAR BIOLOGY, 1987, 7 (02) :679-686
[8]   PHOSPHORYLATION OF INITIATION FACTOR-2-ALPHA BY PROTEIN-KINASE GCN2 MEDIATES GENE-SPECIFIC TRANSLATIONAL CONTROL OF GCN4 IN YEAST [J].
DEVER, TE ;
FENG, L ;
WEK, RC ;
CIGAN, AM ;
DONAHUE, TF ;
HINNEBUSCH, AG .
CELL, 1992, 68 (03) :585-596
[9]   THE NUCLEOTIDE-SEQUENCE OF THE HIS4 REGION OF YEAST [J].
DONAHUE, TF ;
FARABAUGH, PJ ;
FINK, GR .
GENE, 1982, 18 (01) :47-59
[10]  
DRISCOLLPENN M, 1984, MOL CELL BIOL, V4, P520