A MUSCA-DOMESTICA SATELLITE SEQUENCE DETECTS INDIVIDUAL POLYMORPHIC REGIONS IN INSECT GENOME

被引：24

作者：

BLANCHETOT, A

机构：

[1] Department of Biochemistry, University of Saskatchewan, Saskatoon

来源：

NUCLEIC ACIDS RESEARCH | 1991年 / 19卷 / 04期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1093/nar/19.4.929

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A recombinant plasmid, pHMd.24, was constructed which contains an array of a 24 bp sequence from the genome of the Musca domestica. This sequence hybridises to sites occuring at various genomic locations in different housefly strains. The pHMd.24 insert has a structural organisation similar to satellite DNA sequences and hybridises to homologous repeated sequences representing a small fraction of the M. domestica genome. Interestingly, this clone reveals polymorphic fragments from DNA isolated from single houseflies, thus generating an insect DNA fingerprint. In addition, the segregation of the fragments detected by hybridisation in a M. domestica pedigree indicates a transmission that follows mendelian inheritance.

引用

页码：929 / 932

页数：4

共 11 条

[1] DETECTION OF HIGHLY POLYMORPHIC REGIONS IN INSECT GENOMES [J].