DETERMINATION OF DIHYDROSTREPTOMYCIN SULFATE IN MILK BY HPLC USING ION-PAIR AND POSTCOLUMN DERIVATIZATION

被引：19

作者：

HORMAZABAL, V

YNDESTAD, M

机构：

[1] Division of Food Hygiene, Norwegian College of Veterinary Medicine, Oslo, P.O. Box 8146-Dep.

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY | 1995年 / 18卷 / 13期

关键词：

D O I：

10.1080/10826079508009319

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An isocratic, paired-ion reversed-phase high-performance liquid chromatographic method for the determination of dihydrostreptomycin sulfate in milk by postcolumn derivatization has been developed. The milk was treated with 85% trichloroacetic acid to precipitate proteins, and the supernatant clean-up was performed using a tC18 pretreatment column. The correlation coefficients were 0.999. The lower limit of quantification was 25 ng/ml and the limit of detection close to 15 ng/ml. The recovery of dihydrostreptomycin sulfate varying from 82.6 to 82.8%.

引用

页码：2695 / 2702

页数：8

共 14 条

[1]

COX DA, 1977, TOPICS ANTIBIOTIC CH, V1, P5

[2]

FAURE F, 1961, B SOC CHIM BIOL, V43, P953

[3]

GERHARDT GC, 1994, J AOAC INT, V77, P334

[4]

HOLLSTEIN U, 1979, BURGERS MED CHEM 2, P219